Rupintrivir is a promising candidate for treating severe ...
2013-3-1 · Cryosections were prepared using a CM 1800 cryostat (Leica, Wetzlar, Germany) onto poly-l-lysine-coated glass slides, which were further fixed with 3.7% paraformaldehyde in PBS and blocked with 1.5% horse serum in PBS followed by incubation with an anti-EV71 VP1 monoclonal antibody (Millipore, Billerica, MA, USA) at 4 °C overnight. The slides were serially incubated with biotinylated anti-mouse antibody …
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